【成功案例】百迈客云助力喻达辉老师 使用 真核生物有参考基因组的转录组分析平台 辅助研究,发表《通过转录组分析合浦珠母贝对异体血细胞免疫表达相关基因》

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摘要:

合浦珠母贝是在中国普遍养殖的海洋珍珠贝,为了培养珍珠,需要将供体珍珠牡蛎的地幔片用细胞核移植到受体中,而这个移植的幔片可能会被受体贝珍珠贝的免疫系统所拦截,从而降低移植成功率,然而,现在关于珍珠贝对同种异体移植的免疫防御报道很少。在本研究中,对同种异体移植后0小时和48小时的珍珠贝的血细胞免疫反应进行了转录组分析。测序得到92.5M的clean reads,与合浦珠母贝的参考基因组进行了比对,为了鉴定所有与免疫相关的差异表达基因,本研究进行了GO注释和KEGG通路分析。与0小时相比,在移植后48个小时的样本中总共有798个差异表达基因(410个上调基因,388个下调基因),其中血细胞中白细胞介素受体和toll-like受体的表达水平显着升高,表明移植诱导了珍珠贝的免疫应答反应。最后,本研究对其中的18个随机选取的免疫相关的差异表达基因进行了qRT-PCR验证。本文的研究结果为进一步分析同种异体移植的免疫排斥提供了依据。

本文主要用了百迈客云平台主流程。

英文摘要:

The pearl oyster Pinctada fucata is commonly cultured for marine pearls in China. To culture pearls, a mantle piece from a donor pearl oyster is grafted with a nucleus into a receptor. This transplanted mantle piece may be rejected by the immune system of the recipient oyster, thus reducing the success of transplantation. However, there have been limited studies about the oyster’s immune defense against allograft. In this study, hemocyte transcriptome analysis was performed to detect the immune responses to allograft in P. fucata at 0 h and 48 h after a transplant. The sequencing reaction produced 92.5 million reads that were mapped against the reference genome sequences of P. fucata. The Gene Ontology (GO) annotation and the Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to identify all immune- related differentially expressed genes (DEGs). Compared with patterns at 0 h, a total of 798 DEGs were identified, including 410 up-regulated and 388 down-regulated genes at 48 h. The expression levels of interleukin receptor and toll-like receptor in hemocytes were increased significantly 48 h post- transplant, indicating that the oyster immune response was induced. Finally, altered levels of 18 randomly selected immune-related DEGs were confirmed by quantitative real-time PCR (qRT-PCR). Our results provide the basis for further analysis of the immune rejection of allotransplantation.

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