05月03 【成功案例】百迈客云 BLAST小工具 助力 孙青原老师 发表《N6-甲基腺苷测序揭示了mRNA甲基化通过调节非洲爪蟾的翻译水平对卵母细胞减数分裂成熟和胚胎发育的影响》
中文摘要：
在非洲爪蟾的卵母细胞时期，卵母细胞积累了早期胚胎发育的母体材料。由于卵母细胞的转录活性在完全生长阶段沉默，全基因组转录只在中胚层胚胎期才被重新激活，卵母细胞生长期积累的母体mRNA的翻译应该被准确调节。以前的研究结果表明poly（A）尾长度和RNA结合元件介导卵母细胞中的RNA翻译调节，最近发现RNA甲基化存在于各个翻译调节系统中。本研究对完全生长的生殖囊泡（GV）阶段和中期II（MII）期卵母细胞中的N6-甲基腺苷（m6A）修饰的mRNA进行测序。结果，在GV期或MII期卵母细胞中鉴定了具有m6A峰的4,207个mRNA。接着将mRNA甲基化数据与转录组和蛋白组数据结合分析，结果表明，尽管在RNA表达水平没有显著性差异，但是高甲基化的mRNA对应的蛋白质表达水平显著低于低甲基化mRNA的蛋白表达水平，同时还发现低甲基化的mRNA主要富集在细胞周期和翻译途径，而高甲基化的mRNA主要与蛋白磷酸化有关。本文的研究结果表明卵母细胞中的mRNA甲基化可以调节卵母细胞减数分裂成熟和早期胚胎发育过程中的细胞分化和细胞分裂。
本文用了百迈客云平台BLAST比对小工具。
英文摘要：
During the oogenesis of Xenopus laevis, oocytes accumulate maternal materials for early embryo development. As the transcription activity of oocyte is silenced at the fully-grown stage and the global genome is reactivated only by the mid- blastula embryo stage, the translation of maternal mRNAs accumulated during oocyte growth should be accurately regulated. Previous evidence has illustrated that the poly(A) tail length and RNA binding elements mediate RNA translation regulation in oocyte. Recently, RNA methylation is found to exist in various systems. In the present study, we sequenced the N6-methyladenosine (m6A) modified mRNAs in fully-grown germinal vesicle (GV) stage and metaphase II (MII) stage oocytes. As a result, we identified 4207 mRNAs with m6A peaks in the GV stage or MII stage oocytes. When we integrated the mRNA methylation data with transcriptome and proteome data, we found that the highly methylated mRNAs showed significantly lower protein levels than those of the hypomethylated mRNAs, although the RNA levels showed no significant difference. We also found that the hypomethylated mRNAs were
mainly enriched in the cell cycle and translation pathways, whereas the highly methylated mRNAs were mainly associated with the protein phosphorylation. Our results suggest that the oocyte mRNA methylation can regulate the cellular translation and cell division during oocyte meiotic maturation and early embryo development.